Current Nuclear Engineering Strategies in the Green Microalga Chlamydomonas reinhardtii

The green model microalga Chlamydomonas reinhardtii recently emerged as a sustainable production chassis for the efficient biosynthesis of recombinant proteins and high-value metabolites. Its capacity scalable, rapid light-driven growth in minimal salt solutions, its simplicity genetic manipulation “Generally Recognized As Safe” (GRAS) status are key features application industrial biotechnology. Although nuclear transformation has typically resulted limited transgene expression levels, recent developments now allow design powerful innovative bioproduction concepts. In this review, we summarize main obstacles to engineering C. describe all essential aspects sequence adaption vector enable sufficient from genome. Several biotechnological examples successful serve blueprints future establishment cell factory.